Article
High-throughput fluorescent screening of transgenic animals: phenotyping and haplotyping.
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Oct 2006
Hellrung Daniel J, Rossi Gabriela, Link Charles J
Abstract excerpt
BACKGROUND: Methods for genotyping transgenic animals currently consist of extracting genomic DNA from blood or tissue followed by PCR or Southern blot analysis. These methods when used to screen large numbers of animals can be time consuming and expensive. Therefore, we developed a novel method that allows high-throughput screening of phenotypic changes on leukocytes, resulting from the transgenic genotype. This...
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