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Article

SNP discovery in non-model organisms using 454 next generation sequencing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012

Wheat Christopher W

Abstract excerpt

Roche 454 sequencing of the transcriptome has become a standard approach for efficiently obtaining single nucleotide polymorphisms (SNPs) in non-model species. In this chapter, the primary issues facing the development of SNPs from the transcriptome in non-model species are presented: tissue and sampling choices, mRNA preparation, considerations of normalization, pooling and barcoding, how much to sequence, how...

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PMID: 22665274
DOI: 10.1007/978-1-61779-870-2_3

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