The virD genes from the vir region of the Ti plasmid: T-region border dependent processing steps in different rec mutants of Escherichia coli.

doi:10.1016/0378-1119(90)90339-s

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Article

The virD genes from the vir region of the Ti plasmid: T-region border dependent processing steps in different rec mutants of Escherichia coli.

Gene - 30 Nov 1990

Alt-Mörbe J, Heinemeyer W, Schröder J

Abstract excerpt

We evaluated the substrate requirements for virD-mediated T-circle formation in an in vivo binary test system in Escherichia coli. Two copies of the 25-bp sequence which defines the right border of the T-DNA (transferred DNA) are sufficient, and the right and the left copy of the border are equiv...

Topics

Blotting, Southern
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Mutation
Plasmids
Substrate Specificity

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