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Article

Exonuclease requirements for recombination of lambda-phage in recD mutants of Escherichia coli.

Genetics - 1 Aug 2006

Dermić Damir, Zahradka Davor, Petranović Mirjana

Abstract excerpt

Recombination of lambda red gam phage in recD mutants is unaffected by inactivation of RecJ exonuclease. Since nucleases play redundant roles in E. coli, we inactivated several exonucleases in a recD mutant and discovered that 5'-3' exonuclease activity of RecJ and exonuclease VII is essential for lambda-recombination, whereas exonucleases of 3'-5' polarity are dispensable. The implications of the presented data...

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Identifiers

PMID: 16702415
DOI: 10.1534/genetics.106.060426
PMCID: PMC1569708

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Source receipt: pubmed, retrieved 7 Sept 2026.

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