Article
The E288K colon tumor variant of DNA polymerase β is a sequence specific mutator.
Biochemistry - 3 Jul 2012
Murphy Drew L, Donigan Katherine A, Jaeger Joachim, Sweasy Joann B
Abstract excerpt
DNA polymerase β (pol β) is the main polymerase involved in base excision repair (BER), which is a pathway responsible for the repair of tens of thousands of DNA lesions per cell per day. Our recent efforts in sequencing colon tumors showed that 40% of the tumors sequenced possessed a variant in the coding region of the POLB gene; one of these variants is E288K. Expression of the E288K variant in cells leads to...
Topics
- Cell Line
- Circular Dichroism
- Colonic Neoplasms
- DNA Polymerase beta
- Genomic Instability
- Humans
- Mutagenesis
- Mutation