Article
Caenorhabditis elegans screen reveals role of PAR-5 in RAB-11-recycling endosome positioning and apicobasal cell polarity.
Nature cell biology - 27 May 2012
Winter Julia Franziska, Höpfner Sebastian, Korn Kerstin, Farnung Benjamin O, Bradshaw Charles R, Marsico Giovanni, Volkmer Michael, Habermann Bianca, Zerial Marino
Abstract excerpt
Apically enriched Rab11-positive recycling endosomes (Rab11-REs) are important for establishing and maintaining epithelial polarity. Yet, little is known about the molecules controlling trafficking of Rab11-REs in an epithelium in vivo. Here, we report a genome-wide, image-based RNA interference screen for regulators of Rab11-RE positioning and transport of an apical membrane protein (PEPT-1) in C. elegans...
Topics
- Actins
- Animals
- Caenorhabditis elegans
- Caenorhabditis elegans Proteins
- Cell Polarity
- Endosomes
- Fluorescent Antibody Technique
- Genotype
- Intestinal Mucosa
- Microscopy, Confocal
- Microscopy, Fluorescence
- Microtubules
- Peptide Transporter 1