Article
Functional significance of conserved cysteines in the human organic cation transporter 2.
American journal of physiology. Renal physiology - 15 Jul 2012
Pelis Ryan M, Dangprapai Yodying, Cheng Yaofeng, Zhang Xiaohong, Terpstra Jennifer, Wright Stephen H
Abstract excerpt
The significance of conserved cysteines in the human organic cation transporter 2 (hOCT2), namely the six cysteines in the long extracellular loop (loop cysteines) and C474 in transmembrane helix 11, was examined. Uptake of tetraethylammonium (TEA) and 1-methyl-4-phenypyridinium (MPP) into Chinese hamster ovary cells was stimulated >20-fold by hOCT2 expression. Both cell surface expression and transport activity...
Topics
- 1-Methyl-4-phenylpyridinium
- Alanine
- Animals
- Biological Transport
- Cell Membrane
- Cells, Cultured
- Cricetinae
- Cricetulus
- Cysteine
- Female
- Humans
- Mutation
- Organic Cation Transport Proteins
- Organic Cation Transporter 2