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Article

A comprehensive analysis of precursor microRNA cleavage by human Dicer.

RNA (New York, N.Y.) - 1 Nov 2012

Feng Yong, Zhang Xiaoxiao, Graves Paul, Zeng Yan

Abstract excerpt

Dicer cleaves double-stranded RNAs (dsRNAs) or precursor microRNAs (pre-miRNAs) to yield ≈ 22-nt RNA duplexes. The pre-miRNA structure requirement for human Dicer activity is incompletely understood. By large-scale in vitro dicing assays and mutagenesis studies, we showed that human Dicer cleaves most, although not all, of the 161 tested human pre-miRNAs efficiently. The stable association of RNAs with Dicer, as...

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Identifiers

PMID: 22984192
DOI: 10.1261/rna.033688.112
PMCID: PMC3479397

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