Article
[Colorimetric detection of norovirus genotype GII by reverse transcription loop-mediated isothermal amplification].
Bing du xue bao = Chinese journal of virology - 1 Mar 2012
Luo Jian-Ming, Wu Xi-Yang, Xu Zi-Qian, Luo Le, Nie Kai, Yang Meng-Jie, Zeng Ya-Lan, Duan Zhao-Jun, Ma Xue-Jun
Abstract excerpt
A simple, rapid and sensitive colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was established to detect norovirus genotype GII. The method employed a set of six specially designed primers that recognized eight distinct sequences of RNA-dependant RNA polymerase and capsid protein gene for amplification of nucleic acid under isothermal conditions at 65 degrees C for 60...
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