Article
Visual detection of norovirus genogroup II by reverse transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye.
Food and environmental virology - 1 Sept 2014
Luo Jianming, Xu Ziqian, Nie Kai, Ding Xiong, Guan Li, Wang Ji, Xian Yuying, Wu Xiyang, Ma Xuejun
Abstract excerpt
A simple, rapid, specific, and sensitive colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay with hydroxynaphthol blue dye (HNB) was established, targeting RNA-dependent RNA polymerase and capsid protein gene for the detection of the dominant norovirus genogroup in China-NoV GII. The assay was carried out at 65 °C for 60 min with no cross-reactivity with other common...
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