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Article

Real-time molecular beacon NASBA for rapid and sensitive detection of norovirus GII in clinical samples

1 Dec 2009

Abstract excerpt

To improve the sensitivity and efficiency of the real-time nucleic acid sequence based amplification (NASBA) assay targeting the open reading frame 1-2 (ORF1-ORF2) junction of the norovirus (NoV) genome, a selection of clinical samples were analyzed. The assay results were compared with those of TaqMan and conventional reverse transcription PCR (RT-PCR) and a commercial enzyme-linked immunoassay (ELISA) for the...

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PMID: 20029529
DOI: 10.1139/w09-105

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