Article
Analytical performance of norovirus real-time RT-PCR detection protocols in Canadian laboratories.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Feb 2011
Mattison Kirsten, Grudeski Elsie, Auk Brian, Brassard Julie, Charest Hugues, Dust Kerry, Gubbay Jonathan, Hatchette Todd F, Houde Alain, Jean Julie, Jones Tineke, Lee Bonita E, Mamiya Hiroshi, McDonald Ryan, Mykytczuk Oksana, Pang Xiaoli, Petrich Astrid, Plante Daniel, Ritchie Gordon, Wong Julie, Booth Tim F
Abstract excerpt
BACKGROUND: Noroviruses (NoVs) are the leading cause of infectious gastroenteritis worldwide. Real-time reverse transcription PCR (real-time RT-PCR) is the preferred method of NoV detection for the majority of testing laboratories. Although the accepted target region for molecular detection assays is the conserved ORF1/ORF2 junction, multiple variations have been published with differences in primers, probes,...
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