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[Establish A duplex fluorescent quantitative one-step RT-PCR system for the detection of norovirus genogroup I and II].

Bing du xue bao = Chinese journal of virology - 1 May 2013

Zhou Dong-Mei, Jin Miao, Li Hui-Ying, Xu Zi-Qian, Duan Zhao-Jun

Abstract excerpt

The object of this study is to develop a duplex fluorescent quantitative one-step RT-PCR assay for detection and quantitation of GI and GII norovirus. The specific primers, Taqman probes, optimized reaction solution and condition were used to develop the duplex fluorescent quantitative one-step RT-PCR assay. The sensitivity, specificity and reproducibility of the assay were evaluated. The assay was evaluated by...

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PMID: 23905476

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