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Article

Establishment of a Nucleic Acid Detection Method for Norovirus GII.2 Genotype Based on RT-RPA and CRISPR/Cas12a-LFS.

Polish journal of microbiology - 1 Jun 2024

Wang Ting, Zeng Hao, Kang Jie, Lei Lanlan, Liu Jing, Zheng Yuhong, Qian Weidong, Fan Cheng

Abstract excerpt

To establish a rapid detection method for norovirus GII.2 genotype, this study employed reverse transcription recombinase polymerase amplification (RT-RPA) combined with CRISPR/Cas12a and lateral flow strip (RT-RPA-Cas12a-LFS). Here, the genome of norovirus GII.2 genotype was compared to identify highly conserved sequences, facilitating the design of RT-RPA primers and crRNA specific to the conserved regions of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 38905280
DOI: 10.33073/pjm-2024-023
PMCID: PMC11192556

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