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Article

A one-step multiplex real-time RT-PCR assay for rapid and simultaneous detection of human norovirus genogroup I, II and IV.

Journal of virological methods - 1 May 2013

Yan Yong, Wang Heng-hui, Gao Lei, Ji Ji-mei, Ge Zhi-jie, Zhu Xin-qiang, He Pei-yan, Chen Zhong-wen

Abstract excerpt

A one-step multiplex real-time reverse transcription-PCR (RT-PCR) assay was developed for one-tube and simultaneous detection of three genogroups of human norovirus, genogroup I, II and IV (GI, GII and GIV). The specificity and sensitivity of the assay were evaluated and 50 samples were tested by using this assay. The results showed that the multiplex assay had high sensitivity and specificity. The amplification...

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Identifiers

PMID: 23454645
DOI: 10.1016/j.jviromet.2013.02.004

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