Article
Kinetics and thermodynamics of triple-helix formation: effects of ionic strength and mismatches.
Biochemistry - 29 Sept 1992
Rougée M, Faucon B, Mergny J L, Barcelo F, Giovannangeli C, Garestier T, Hélène C
Abstract excerpt
Thermodynamic and kinetic parameters for the triplex-forming reactions between a homopurine-homopyrimidine 22-base-pair duplex (sequence of the purine strand: 5'd[AAAGGAGGAGAAGAAGAAAAAA]3') and the four 22-dN third strands (22 dN: 5'd[TTTCCTCCTCTNCTTCTTTTTT]3', where N = A, C, T, or G) were determined from thermal denaturation and renaturation UV absorbance profiles. Cooling and heating curves were not...
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