Article
Sequence specificity in triple-helix formation: experimental and theoretical studies of the effect of mismatches on triplex stability.
Biochemistry - 8 Oct 1991
Mergny J L, Sun J S, Rougée M, Montenay-Garestier T, Barcelo F, Chomilier J, Hélène C
Abstract excerpt
The specificity of a homopyrimidine oligonucleotide binding to a homopurine-homopyrimidine sequence on double-stranded DNA was investigated by both molecular modeling and thermal dissociation experiments. The presence of a single mismatched triplet at the center of the triplex was shown to destabilize the triple helix, leading to a lower melting temperature and a less favorable energy of interaction. A terminal...
Topics
- Base Composition
- Base Sequence
- DNA
- Hot Temperature
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Spectrophotometry, Ultraviolet
- Structure-Activity Relationship