Article
Fast homozygosity mapping and identification of a zebrafish ENU-induced mutation by whole-genome sequencing.
PloS one - 1 Jan 2012
Voz Marianne L, Coppieters Wouter, Manfroid Isabelle, Baudhuin Ariane, Von Berg Virginie, Charlier Carole, Meyer Dirk, Driever Wolfgang, Martial Joseph A, Peers Bernard
Abstract excerpt
Forward genetics using zebrafish is a powerful tool for studying vertebrate development through large-scale mutagenesis. Nonetheless, the identification of the molecular lesion is still laborious and involves time-consuming genetic mapping. Here, we show that high-throughput sequencing of the whole zebrafish genome can directly locate the interval carrying the causative mutation and at the same time pinpoint the...
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