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Excited state proton transfer in strongly enhanced GFP (sGFP2).

Physical chemistry chemical physics : PCCP - 7 Jul 2012

van Oort Bart, ter Veer Mirelle J T, Groot Marie Louise, van Stokkum Ivo H M

Abstract excerpt

Proton transfer is an elementary process in biology. Green fluorescent protein (GFP) has served as an important model system to elucidate the mechanistic details of this reaction, because in GFP proton transfer can be induced by light absorption. We have used pump-dump-probe spectroscopy to study how proton transfer through the 'proton-wire' around the chromophore is affected by a combination of mutations in a...

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Escherichia coli
Green Fluorescent Proteins
Microscopy
Mutation
Protons

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PMID: 22473040
DOI: 10.1039/c2cp40694b

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