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Article

Highly efficient selection of the stable clones expressing antibody-IL-2 fusion protein by a dicistronic expression vector containing a mutant neo gene.

Journal of immunological methods - 1 Dec 2004

Chen Liyong, Xie Zhigang, Teng Yan, Wang Minggao, Shi Ming, Qian Lu, Hu Meiru, Feng Jiannan, Yang Xiao, Shen Beifen, Guo Ning

Abstract excerpt

To increase the selection efficiency and productivity of stable clones expressing recombinant antibody-IL-2 fusion protein, a dicistronic expression vector containing the anti-erbB2 scFv-Fc-IL-2 fusion gene followed by a weakened neo gene, was constructed to allow for the concurrent translation of the recombinant protein and mutated neomycin phosphotransferase from a single mRNA. The presence of the mutant enzyme...

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PMID: 15627610
DOI: 10.1016/j.jim.2004.08.013

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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