Article
[The mutation of anti-CD3 antibody (HIT3a) gene and its expression].
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 May 2001
Xu Y, Xiong D, Yang C, Lai Z, Liu H, He X, Peng H, Shao X, Xu C, Liao X, Xie Y, Zhu Z
Abstract excerpt
OBJECTIVE: To improve the expression of anti-CD(3) single chain Fv (scFv) by site mutation and identify its biological activity. METHODS: Anti-CD(3) scFv gene was mutated by PCR, the target clones were screened by both the fingerprints of DNA restriction endonuclease digestion and Western blot, the antigen-binding activity of scFv was examined by FACS, competitive inhibition was performed with (125)I-labeled...
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