Article
Targeting Human α-Lactalbumin Gene Insertion into the Goat β-Lactoglobulin Locus by TALEN-Mediated Homologous Recombination.
PloS one - 1 Jan 2016
Zhu Hongmei, Liu Jun, Cui Chenchen, Song Yujie, Ge Hengtao, Hu Linyong, Li Qian, Jin Yaping, Zhang Yong
Abstract excerpt
Special value of goat milk in human nutrition and well being is associated with medical problems of food allergies which are caused by milk proteins such as β-lactoglobulin (BLG). Here, we employed transcription activator-like effector nuclease (TALEN)-assisted homologous recombination in goat fibroblasts to introduce human α-lactalbumin (hLA) genes into goat BLG locus. TALEN-mediated targeting enabled isolation...
Topics
- Alleles
- Animals
- Female
- Fibroblasts
- Goats
- Homologous Recombination
- Humans
- Lactalbumin
- Lactoglobulins
- Milk
- Mutagenesis, Insertional
- Nuclear Transfer Techniques
- Ovary
- Plasmids