Article
DNA nucleic acid sequence-based amplification-based genotyping for polymorphism analysis.
BioTechniques - 1 Oct 2004
Berard Cecile, Cazalis Marie-Angélique, Leissner Philippe, Mougin Bruno
Abstract excerpt
Nucleic acid sequence-based amplification (NASBA) is a sensitive isothermal transcription-based amplification method known to be a suitable tool for RNA research. We demonstrate that NASBA technology can be applied to single nucleotide polymorphism (SNP) analysis using human genomic DNA as a template. Combination of DNA NASBA with multiplex hybridization of specific molecular beacons makes it possible to...
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