Isolation and characterization of mutants of firefly luciferase which produce different colors of light.

doi:10.1093/protein/4.6.691

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Article

Isolation and characterization of mutants of firefly luciferase which produce different colors of light.

Protein engineering - 1 Aug 1991

Kajiyama N, Nakano E

Abstract excerpt

The luciferase cDNA from the 'Genji' firefly, Luciola cruciata, was mutated with hydroxylamine to isolate mutant luciferases. Some of the isolated mutant enzymes produced different colors of light, ranging from green to red. Five such mutants, producing green (lambda max = 558 nm), yellow-orange...

Topics

Amino Acid Sequence
Animals
Coleoptera
Color
Light
Luciferases
Luminescent Measurements
Mutation
Spectrum Analysis

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