Article
Isolation and characterization of mutants of firefly luciferase which produce different colors of light.
Protein engineering - 1 Aug 1991
Kajiyama N, Nakano E
Abstract excerpt
The luciferase cDNA from the 'Genji' firefly, Luciola cruciata, was mutated with hydroxylamine to isolate mutant luciferases. Some of the isolated mutant enzymes produced different colors of light, ranging from green to red. Five such mutants, producing green (lambda max = 558 nm), yellow-orange (lambda max = 595 nm), orange (lambda max = 607 nm) and red light (lambda max = 609 and 612 nm), were analyzed. The...
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