Article
In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches.
Plant biotechnology journal - 1 Jun 2012
Lorieux Mathias, Blein Mélisande, Lozano Jaime, Bouniol Mathieu, Droc Gaétan, Diévart Anne, Périn Christophe, Mieulet Delphine, Lanau Nadège, Bès Martine, Rouvière Claire, Gay Céline, Piffanelli Pietro, Larmande Pierre, Michel Corinne, Barnola Isabelle, Biderre-Petit Corinne, Sallaud Christophe, Perez Pascual, Bourgis Fabienne, Ghesquière Alain, Gantet Pascal, Tohme Joe, Morel Jean Benoit, Guiderdoni Emmanuel
Abstract excerpt
We report here the molecular and phenotypic features of a library of 31,562 insertion lines generated in the model japonica cultivar Nipponbare of rice (Oryza sativa L.), called Oryza Tag Line (OTL). Sixteen thousand eight hundred and fourteen T-DNA and 12,410 Tos17 discrete insertion sites have been characterized in these lines. We estimate that 8686 predicted gene intervals--i.e. one-fourth to one-fifth of the...
Topics
- DNA, Bacterial
- DNA, Plant
- Gene Library
- Genes, Plant
- Magnaporthe
- Mutagenesis, Insertional
- Oryza
- Phenotype