A rice gene activation/knockout mutant resource for high throughput functional genomics.

doi:10.1007/s11103-006-9093-z

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Article

A rice gene activation/knockout mutant resource for high throughput functional genomics.

Plant molecular biology - 1 Feb 2007

Hsing Yue-Ie, Chern Chyr-Guan, Fan Ming-Jen, Lu Po-Chang, Chen Ku-Ting, Lo Shuen-Fang, Sun Peng-Kai, Ho Shin-Lon, Lee Kuo-Wei, Wang Yi-Chieh, Huang Wen-Lii, Ko Swee-Suak, Chen Shu, Chen Jyh-Long, Chung Chun-I, Lin Yao-Cheng, Hour Ai-Ling, Wang Yet-Walt, Chang Ya-Chi, Tsai Min-Wei, Lin Yi-Show, Chen Yin-Chin, Yen Hsing-Mu, Li Charng-Pei, Wey Chiu-Kai, Tseng Ching-Shan, Lai Ming-Hsing, Huang Sheng-Chung, Chen Liang-Jwu, Yu Su-May

Abstract excerpt

Using transfer DNA (T-DNA) with functions of gene trap and gene knockout and activation tagging, a mutant population containing 55,000 lines was generated. Approximately 81% of this population carries 1-2 T-DNA copies per line, and the retrotransposon Tos17 was mostly inactive in this population during tissue culture. A total of 11,992 flanking sequence tags (FSTs) have been obtained and assigned to the rice...

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PMID: 17120135
DOI: 10.1007/s11103-006-9093-z

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