Article
Large-scale characterization of Tos17 insertion sites in a rice T-DNA mutant library.
Plant molecular biology - 1 Nov 2007
Piffanelli Pietro, Droc Gaétan, Mieulet Delphine, Lanau Nadège, Bès Martine, Bourgeois Emmanuelle, Rouvière Claire, Gavory Fréderick, Cruaud Corinne, Ghesquière Alain, Guiderdoni Emmanuel
Abstract excerpt
We characterized the insertion sites of newly transposed copies of the tissue-culture-induced ty1-copia retrotransposon Tos17 in the Oryza Tag Line (OTL) T-DNA mutant library of rice cv. Nipponbare. While Nipponbare contains two native copies of Tos17 the number of additional copies, deduced from Southern blot analyses in a subset of 384 T-DNA lines and using a reverse transcriptase probe specific to the element,...
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