Rapid identification of Arabidopsis insertion mutants by non-radioactive detection of T-DNA tagged genes.

doi:10.1046/j.1365-313x.2002.01416.x

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Article

Rapid identification of Arabidopsis insertion mutants by non-radioactive detection of T-DNA tagged genes.

The Plant journal : for cell and molecular biology - 1 Oct 2002

Ríos Gabino, Lossow Andrea, Hertel Britta, Breuer Frank, Schaefer Sabine, Broich Melanie, Kleinow Tatjana, Jásik Ján, Winter Jochen, Ferrando Alejandro, Farrás Rosa, Panicot Mireia, Henriques Rossana, Mariaux Jean-Baptist, Oberschall Attila, Molnár Gergely, Berendzen Kenneth, Shukla Vijaya, Lafos Marcel, Koncz Zsuzsanna, Rédei George P, Schell Jeff, Koncz Csaba

Abstract excerpt

To assist in the analysis of plant gene functions we have generated a new Arabidopsis insertion mutant collection of 90 000 lines that carry the T-DNA of Agrobacterium gene fusion vector pPCV6NFHyg. Segregation analysis indicates that the average frequency of insertion sites is 1.29 per line, predicting about 116 100 independent tagged loci in the collection. The average T-DNA copy number estimated by Southern...

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PMID: 12383089
DOI: 10.1046/j.1365-313x.2002.01416.x

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