Functional characterization of rice genes using a gene-indexed T-DNA insertional mutant population.

doi:10.1007/978-1-62703-194-3_5

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Article

Functional characterization of rice genes using a gene-indexed T-DNA insertional mutant population.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Jung Ki-Hong, An Gynheung

Abstract excerpt

Despite the availability of the finished genome sequence and tools for its analyses, few rice genes have been characterized. Because Agrobacterium-mediated transformation causes random T-DNA insertions across the genome, T-DNA can be a good mutagen for functional genomics. Gene-indexed mutants with flanking sequences around inserted T-DNA are valuable resources for accelerating functional characterizations of...

Topics

DNA, Bacterial
Gene Expression Regulation, Plant
Genes, Plant
Genotype
Multigene Family
Mutagenesis, Insertional
Organ Specificity
Oryza
Phenotype
Plants, Genetically Modified

Source

Open in PubMed

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PMID: 23135844
DOI: 10.1007/978-1-62703-194-3_5

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