Article
Dictyostelium myosin heavy chain phosphorylation sites regulate myosin filament assembly and localization in vivo.
Cell - 22 Oct 1993
Egelhoff T T, Lee R J, Spudich J A
Abstract excerpt
Three threonine residues in the tail region of Dictyostelium myosin II heavy chain have been implicated previously in control of myosin filament formation. Here we report the in vitro and in vivo consequences of converting these sites to alanine residues, which eliminates phosphorylation at these positions, or to aspartate residues, which mimics the negative charge state of the phosphorylated molecule. Alanine...
Topics
- Actin Cytoskeleton
- Animals
- Cell Compartmentation
- Cell Division
- Concanavalin A
- Cytoskeleton
- Dictyostelium
- Membrane Proteins
- Mutation
- Myosins
- Octoxynol
- Phosphorylation