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Article

Single-molecule FRET reveals hidden complexity in a protein energy landscape.

Structure (London, England : 1993) - 6 Jan 2015

Tsytlonok Maksym, Ibrahim Shehu M, Rowling Pamela J E, Xu Wenshu, Ruedas-Rama Maria J, Orte Angel, Klenerman David, Itzhaki Laura S

Abstract excerpt

Here, using single-molecule FRET, we reveal previously hidden conformations of the ankyrin-repeat domain of AnkyrinR, a giant adaptor molecule that anchors integral membrane proteins to the spectrin-actin cytoskeleton through simultaneous binding of multiple partner proteins. We show that the ankyrin repeats switch between high-FRET and low-FRET states, controlled by an unstructured "safety pin" or "staple" from...

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Identifiers

PMID: 25565106
DOI: 10.1016/j.str.2014.10.023
PMCID: PMC4291146

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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