Characterization of temperature-sensitive cytidine triphosphate synthase mutations in bacteria by high-performance liquid chromatography.

doi:10.1016/s0021-9673(01)89506-6

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Article

Characterization of temperature-sensitive cytidine triphosphate synthase mutations in bacteria by high-performance liquid chromatography.

Journal of chromatography - 20 Jul 1990

Bailey A J, Dutta P K, Shanley M S, O'Donovan G A

Abstract excerpt

Cytidine triphosphate (CTP) synthase catalyzes the last step in pyrimidine ribonucleotide synthesis, namely the formation of CTP from UTP, ATP, and glutamine. Mutants devoid of CTP synthase activity require cytidine for growth and have been designated pyrG in an obligate cdd background. Using a t...

Topics

Bacteria
Carbon-Nitrogen Ligases
Chromatography, High Pressure Liquid
Cytidine Triphosphate
Indicators and Reagents
Ligases
Mutation
Salmonella typhimurium
Temperature
Uridine Triphosphate

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