Article
Detection of single-nucleotide polymorphisms with novel leaky surface acoustic wave biosensors, DNA ligation and enzymatic signal amplification.
Biosensors & bioelectronics - 15 Mar 2012
Xu Qinghua, Chang Kai, Lu Weiping, Chen Wei, Ding Yi, Jia Shuangrong, Zhang Kejun, Li Fake, Shi Jianfeng, Cao Liang, Deng Shaoli, Chen Ming
Abstract excerpt
This manuscript describes a new technique for detecting single-nucleotide polymorphisms (SNPs) by integrating a leaky surface acoustic wave (LSAW) biosensor, enzymatic DNA ligation and enzymatic signal amplification. In this technique, the DNA target is hybridized with a capture probe immobilized on the surface of a LSAW biosensor. Then, the hybridized sequence is ligated to biotinylated allele-specific detection...
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