Article
Insight into the common mechanism of the chromophore formation in the red fluorescent proteins: the elusive blue intermediate revealed.
Journal of the American Chemical Society - 8 Feb 2012
Bravaya Ksenia B, Subach Oksana M, Korovina Nadezhda, Verkhusha Vladislav V, Krylov Anna I
Abstract excerpt
Understanding the chromophore maturation process in fluorescent proteins is important for the design of proteins with improved properties. Here, we present the results of electronic structure calculations identifying the nature of a blue intermediate, a key species in the process of the red chromophore formation in DsRed, TagRFP, fluorescent timers, and PAmCherry. The chromophore of the blue intermediate has a...
Topics
- Luminescent Proteins
- Models, Molecular
- Molecular Structure
- Mutation
- Quantum Theory
- Red Fluorescent Protein