Article
Use of DNA amplification (PCR) and direct DNA sequencing in the characterization of C4 alleles.
Annals of human genetics - 1 Jul 1990
Berg E S, Markussen G, Teisberg P, Olaisen B
Abstract excerpt
A procedure for detailed characterization of individual C4 alleles has been developed. DNA containing the two polymorphic clusters of C4 was amplified in the polymerase chain reaction (PCR). Direct DNA sequencing of amplified DNA was then performed by a modification of previously described techniques. The results were confirmed by M13 sequencing. Single C4A3 and C4B1 allele sequences were in accordance with...
Topics
- Alleles
- Base Sequence
- Cloning, Molecular
- Complement C4
- DNA
- Evaluation Studies as Topic
- Gene Amplification
- Haplotypes
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction