Article
High-throughput analysis of the C4 polymorphism by a combination of MLPA and isotype-specific ELISA's.
Molecular immunology - 1 Feb 2009
Wouters Diana, van Schouwenburg Pauline, van der Horst Anneke, de Boer Martin, Schooneman Dennis, Kuijpers Taco W, Aarden Lucien A, Hamann Dörte
Abstract excerpt
Complement factor C4 exists as two main isotypes, C4A and C4B, with different functional properties and encoded by two separate genes. In addition, C4A and C4B genes can occur in multiple copies and may carry a retroviral HERV-K(C4) insertion in intron 9. To study association of C4 polymorphism with disease, accurate genotyping and phenotyping is important. However, current techniques are very laborious and not...
Topics
- Adult
- Amplified Fragment Length Polymorphism Analysis
- Bacteria
- Child
- Complement C4a
- Complement C4b
- Endogenous Retroviruses
- Enzyme-Linked Immunosorbent Assay
- Exons
- Gene Dosage
- Genetic Predisposition to Disease
- Genotype
- Humans
- Introns