Article
Antisense RNA inhibition of HPRT synthesis.
Somatic cell and molecular genetics - 1 Jul 1990
Stout J T, Caskey C T
Abstract excerpt
The abundant production of antisense hypoxanthine phosphoribosyltransferase (HPRT) RNA in NIH-3T3, COS, or HeLa cells leads to an inhibition of HPRT synthesis. HPRT enzyme levels in cells transfected with mouse HPRT antisense RNA expression vectors are reduced to less than 1% of parental cell activity, resulting in resistance to 6-thioguanine (6TG). The expression of antisense HPRT RNA leads to a marked reduction...
Topics
- Animals
- Base Sequence
- Cell Line
- Chromosome Deletion
- Drug Resistance
- Exons
- Gene Expression Regulation, Enzymologic
- Gentamicins
- Hypoxanthine Phosphoribosyltransferase
- Introns
- Molecular Sequence Data