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Article

The use of antisense oligonucleotides in Xenopus oocytes.

Methods (San Diego, Calif.) - 1 May 2010

Hulstrand Alissa M, Schneider Patricia N, Houston Douglas W

Abstract excerpt

The ability to manipulate gene expression in Xenopus oocytes and then generate fertilized embryos by transfer into host females has made it possible to rapidly characterize maternal signaling pathways in vertebrate development. Maternal mRNAs in particular can be efficiently depleted using antisense deoxyoligonucleotides (oligos), mediated by endogenous RNase-H activity. Since the microinjection of antisense...

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Identifiers

PMID: 20045732
DOI: 10.1016/j.ymeth.2009.12.015
PMCID: PMC2868083

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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