Article
DNA polymerase II is encoded by the DNA damage-inducible dinA gene of Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America - 1 Oct 1990
Bonner C A, Hays S, McEntee K, Goodman M F
Abstract excerpt
The structural gene for DNA polymerase II was cloned by using a synthetic inosine-containing oligonucleotide probe corresponding to 11 amino acids, which were determined by sequencing the amino terminus of the purified protein. The labeled oligonucleotide hybridized specifically to the lambda clone 7H9 from the Kohara collection as well as to plasmid pGW511 containing the SOS-regulated dinA gene. Approximately...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Damage
- DNA Polymerase II
- Enzyme Induction
- Escherichia coli
- Genes, Bacterial
- Humans
- Molecular Sequence Data
- Mutation