Article
Detection of heteroduplex DNA molecules among the products of Saccharomyces cerevisiae meiosis.
Proceedings of the National Academy of Sciences of the United States of America - 1 Oct 1990
Lichten M, Goyon C, Schultes N P, Treco D, Szostak J W, Haber J E, Nicolas A
Abstract excerpt
We have used denaturant-gel electrophoresis to provide a physical demonstration of heteroduplex DNA in the products of yeast meiosis. We examined heteroduplex formation at arg4-nsp, a G.C----C.G transversion that displays a moderately high level of postmeiotic segregation. Of the two possible arg...
Topics
- Base Sequence
- Crosses, Genetic
- DNA, Fungal
- Genotype
- Meiosis
- Molecular Sequence Data
- Nucleic Acid Heteroduplexes
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Plasmids
- Saccharomyces cerevisiae
- Spores, Fungal