Article
Extensive 3'-overhanging, single-stranded DNA associated with the meiosis-specific double-strand breaks at the ARG4 recombination initiation site.
Cell - 22 Mar 1991
Sun H, Treco D, Szostak J W
Abstract excerpt
Meiosis-specific double-strand breaks occur at the initiation site for meiotic gene conversion in the yeast ARG4 gene. Here we show that the break fragments end in extensive 3'-overhanging, single-stranded tails. The single-stranded tails very in length, generating a gradient of single-strandedness that parallels the gradient of gene conversion frequencies in ARG4. In strains carrying a rad50S mutation, which...
Topics
- DNA, Fungal
- DNA, Single-Stranded
- Gene Conversion
- Genes, Fungal
- Meiosis
- Mutation
- Recombination, Genetic
- Saccharomyces cerevisiae