Article
U1 small nuclear RNA plays a direct role in the formation of a rev-regulated human immunodeficiency virus env mRNA that remains unspliced.
Proceedings of the National Academy of Sciences of the United States of America - 1 Oct 1990
Lu X B, Heimer J, Rekosh D, Hammarskjöld M L
Abstract excerpt
rev-regulated expression of HIV-1 envelope proteins from a simian virus 40 late replacement vector was found to be dependent on the presence of a 5' splice site in the env mRNA in spite of the fact that this mRNA remains unspliced. When the 5' splice site upstream of the env open reading frame was deleted or mutated, expression of envelope protein was lost. RNA analysis of cells transfected with 5' splice-site...
Topics
- Animals
- Base Sequence
- Cell Line
- Chromosome Deletion
- Gene Expression Regulation, Viral
- Gene Products, rev
- Genes, Viral
- HIV-1
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes