Distinct RNA sequences in the gag region of human immunodeficiency virus type 1 decrease RNA stability and inhibit expression in the absence of Rev protein.

Abstract excerpt

The expression of Gag, Pol, Vif, Vpr, Vpu, and Env proteins from unspliced and partially spliced human immunodeficiency virus type 1 (HIV-1) mRNAs depends on the viral protein Rev, while the production of Tat, Rev, and Nef from multiply spliced mRNAs does not require Rev. To investigate the difference between gag and tat mRNAs, we generated plasmids expressing tat-gag hybrid mRNAs. Insertion of the gag gene...

Topics

• Blotting, Northern
• Blotting, Western
• Cell Line
• Cloning, Molecular
• Gene Expression Regulation, Viral
• Gene Products, rev
• Gene Products, tat
• Genes, gag
• Genes, tat
• HIV-1
• Humans