Comparison of Illumina paired-end and single-direction sequencing for microbial 16S rRNA gene amplicon surveys

Abstract excerpt

High-throughput sequencing of 16S rRNA gene amplicons is a valuable tool for comparing microbial community structure among hundreds of samples for which researchers have primarily used the 454 Pyrosequencing platform. The Illumina platform produces far more reads than 454 (up to 1.5 billion reads per run, compared with 1 million reads per run on a 454 plate of comparable cost), but produces fewer base pairs (bp)...

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