Article
Genes A27L and F13L as Genetic Markers for the Isolation of Recombinant Vaccinia Virus.
Scientific reports - 30 Oct 2019
Lorenzo María M, Sánchez-Puig Juana M, Blasco Rafael
Abstract excerpt
After assembly in the cytosol, some Vaccinia virus particles go through a complex process that leads to virus egress and eventually cell-to-cell transmission. Intracellular particles are fully infectious, and therefore virus mutants lacking essential functions in the exit pathway are unable to form plaques but can multiply intracellularly. We isolated virus mutants in which two of the genes required for virus...
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