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Article

Humanization of antibodies using heavy chain complementarity-determining region 3 grafting coupled with in vitro somatic hypermutation.

The Journal of biological chemistry - 15 Mar 2013

Bowers Peter M, Neben Tamlyn Y, Tomlinson Geoffery L, Dalton Jennifer L, Altobell Larry, Zhang Xue, Macomber John L, Wu Betty F, Toobian Rachelle M, McConnell Audrey D, Verdino Petra, Chau Betty, Horlick Robert A, King David J

Abstract excerpt

A method for simultaneous humanization and affinity maturation of monoclonal antibodies has been developed using heavy chain complementarity-determining region (CDR) 3 grafting combined with somatic hypermutation in vitro. To minimize the amount of murine antibody-derived antibody sequence used during humanization, only the CDR3 region from a murine antibody that recognizes the cytokine hβNGF was grafted into a...

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Share to Antibodies
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Binding, Competitive

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Identifiers

PMID: 23355464
DOI: 10.1074/jbc.M112.445502
PMCID: PMC3597809

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