Article
Retrocyte Display® technology: generation and screening of a high diversity cellular antibody library.
Methods (San Diego, Calif.) - 1 Jan 2014
Breous-Nystrom Ekaterina, Schultze Kornelia, Meier Marco, Flueck Lukas, Holzer Christina, Boll Melanie, Seibert Volker, Schuster Andrea, Blanusa Milan, Schaefer Verena, Grawunder Ulf, Martin-Parras Luis, van Dijk Marc A
Abstract excerpt
Over the last nearly three decades in vitro display technologies have played an important role in the discovery and optimization of antibodies and other proteins for therapeutic applications. Here we describe the use of retroviral expression technology for the display of full-length IgG on B lineage cells in vitro with a hallmark of a tight and stable genotype to phenotype coupling. We describe the creation of a...
Topics
- Animals
- Antibodies, Monoclonal
- B-Lymphocytes
- Cryopreservation
- Drug Evaluation, Preclinical
- Flow Cytometry
- Genetic Variation
- Genetic Vectors
- HEK293 Cells
- Humans
- Immunoglobulin Heavy Chains
- Immunoglobulin Light Chains