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Article

Further stabilization of Leu¹⁵⁵ mutant thermolysins by mutation of an autodegradation site.

Applied biochemistry and biotechnology - 1 Feb 2012

Matsumiya Yoshiki, Murata Naoko, Inouye Kuniyo, Kubo Motoki

Abstract excerpt

The autodegradation-resistant mutant thermolysins (TLNs), L155A (Leu(155) to Ala) and L155S (Leu(155) to Ser), were previously constructed by site-directed mutagenesis to enhance thermostability. These mutations suppressed autodegradation at position 154-155, resulting in increased thermostability. However, a new autodegradation site became apparent in these mutant TLNs, at position 155-156. In this study,...

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PMID: 22139731
DOI: 10.1007/s12010-011-9462-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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