Article
Construction of a plasmid used for the expression of a sevenfold-mutant barley beta-amylase with increased thermostability in Escherichia coli and properties of the sevenfold-mutant beta-amylase.
Journal of biochemistry - 1 Sept 1995
Yoshigi N, Okada Y, Maeba H, Sahara H, Tamaki T
Abstract excerpt
To increase the thermostability of beta-amylase, seven kinds of single-mutant plasmids were constructed with an expression vector of barley beta-amylase by mutagenesis. The remaining activity versus temperature curves were used to determine the temperatures (T50) at which 50% of the initial activ...
Topics
- Base Sequence
- Electrophoresis, Polyacrylamide Gel
- Enzyme Inhibitors
- Enzyme Stability
- Escherichia coli
- Genetic Vectors
- Hordeum
- Metals
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plant Proteins
- Plasmids
- Substrate Specificity
- Temperature
- beta-Amylase