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Article

Effects of the mutational combinations on the activity and stability of thermolysin.

Journal of biotechnology - 3 May 2010

Kusano Masayuki, Yasukawa Kiyoshi, Inouye Kuniyo

Abstract excerpt

We have previously indicated that three single mutations (Leu144-->Ser, Asp150-->Glu, and Ile168-->Ala) in the site-directed mutagenesis of thermolysin increase the activity and two single (Ser53-->Asp and Leu155-->Ala) and one triple (Gly8-->Cys/Asn60-->Cys/Ser65-->Pro) mutations increase the stability. In the present study, aiming to generate highly active and stable thermolysin variants, we combined these...

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PMID: 20214932
DOI: 10.1016/j.jbiotec.2010.02.024

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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